Technical Topics Podcast
1) Leading the Pack with Molecular Quantitation on the LightCycler® 2.0 Instrument
Areas of discussion include Chromosome Structure, Workflow of molecular testing, Biological Sample, Building a Real-Time PCR Reaction, Absolute Quantification Analysis, Automated Quantification Analys...Show More
Leading the Pack with Molecular Quantitation on the LightCycler® 2.0 Instrument
11:57 | Jul 18th, 2012
2) Molecular Stats: Prevalence and Positive Predictive Value
Overview of how disease prevalence effects the concept of positive predictive value of a diagnostic test and also how changes in performance characteristics can affect the positive predictive value.
3) Molecular Stats: Limit of Detection & Limit of Quantification
Overview of Limit of Detection (LoD) and Limit of Quantification (LoQ) as important analytical measurements in the performance of molecular assay
4) Molecular Stats: Sensitivity and Specificity
Sensitivity and Specificity are analytical methods that help determine the performance of a laboratory test.
5) Calculating Molarity for PCR Reactions
The fundamentals of the polymerase chain reaction (PCR) and how to calculate the specific concentration or molarity of the reaction elements required to perform a PCR reaction. Speaker: Art Braden, ...Show More
6) Molecular Stats: Accuracy and Precision
An overview of the theoretical application of Accuracy and Precision in the validation of a Laboratory Assay and a practical example.
7) Molecular Stats: Correlation and Method Comparison
Method comparisons are a way to determine if a new method can replace an old method by confirming that the two tests sufficiently agree. This agreement is assessed based on visual and statistical para...Show More